Nitric Oxide Protocols

2. Auflage 2004
ISBN: 978-1-61737-443-2
Verlag: Humana Press

Buch, Englisch, Band 279, 248 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 386 g

Reihe: Methods in Molecular Biology

It has been more than five years since the appearance of the first edition of Nitric Oxide Protocols. According to Medline, more than 32,500 publications dealing with nitric oxide have appeared in the primary literature in the int- vening time from 1999 to April 2004. These numbers attest to the continuing vitality of a field dealing with a deceptively simple, although extremely int- esting and promiscuous molecule, nitric oxide. Despite its apparent chemical simplicity, nitric oxide continues to amaze, with an extremely wide spectrum of functions and effects. In particular, the last few years have witnessed an explosion of nonclassical effects, involving the direct interaction of nitric - ide and related oxidized products with proteins to yield such novel combi- tions as nitrosylated and nitrated proteins. Another emergent area has been that of NO’s clinical applications, including interesting developments related to the virus-mediated expression of nitric oxide synthase and the use of nitric oxide in gene therapy. In Nitric Oxide Protocols, I have gathered chapters dealing with the some of the latest methodologies for the measurement of nitric oxide. Many of these deal with quantitation in biological fluids or tissues, and they cover a wide spectrum of technologies, including capillary electrophoresis, microcoaxial electrodes, the in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography–mass spectroscopy, in situ hybridization, and real-time polymerase chain reaction.

Zielgruppe

Research

Autoren/Hrsg.

Hassid, Aviv

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO.- High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples.- Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis.- In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode.- Measurement of Exhaled Nitric Oxide.- Nitric Oxide Imaging in Neurons Using Confocal Microscopy.- Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry.- A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts.- Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization.- Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase.- Dioxygen-Dependent Metabolism of Nitric Oxide.- Proteomic Method for Identification of Tyrosine-Nitrated Proteins.- Immunohistochemical Detection of S-Nitrosylated Proteins.- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction.- Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats.- Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase.- Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors.- Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer.