Gómez / Buscaglia T. cruzi Infection

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9148-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1955, 398 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Gómez, Karina Andrea

Buscaglia, Carlos Andrés

Inhaltsverzeichnis

Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome.- Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development.- Transcriptome Studies in Trypanosoma cruzi using RNAseq.- An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene.- Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi.- Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes.- Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi.- Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae.- Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi.- Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase.- Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions.- Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues.- In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters.- Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models.- MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice.- Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR.- Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR.- Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase.- Analytics of Functional Autoantibodies in Patients with Chagas’ Disease.- Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease.- Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma.- Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease.- Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients.- Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients.- Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease.- CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease.- Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease.- Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts.