Fox / Bierne / Stessl Listeria Monocytogenes

Methods and Protocols
2. Auflage 2021
ISBN: 978-1-0716-0982-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2220, 260 Seiten, eBook

Reihe: Methods in Molecular Biology

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of
L. monocytogenes
, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate
Listeria
, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls. 

Authoritative and up-to-date,
Listeria monocytogenes: Methods and Protocols, Second Edition
aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to
Listeria
research both in relation to food association and control as well as clinical microbiology.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Fox, Edward M.

Bierne, Hélène

Stessl, Beatrix

Inhaltsverzeichnis

Traditional Methods of Analysis for
Listeriamonocytogenes
.- MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping
Listeria monocytogenes
.- Sample Preparation for qPCR Detection of
Listeria
from Food.- qPCR Validation on the Basis of the
Listeria monocytogenesprfA
Assay.- Serotype Assignment by Sero-Agglutination, ELISA, and PCR.- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of
Listeria monocytogenes
.- Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of
Listeria monocytogenes
and
Listeria innocua
.- High-Throughput Characterization of
Listeria monocytogenes
Using the OmniLog Phenotypic Microarray.- High-Throughput Screening of Biofilm Formation of 
Listeria monocytogenes
 on Stainless Steel Coupons Using a 96-Well Plate Format.- Confocal Laser Microscopy Analysis of
Listeria monocytogenes
Biofilms and Spatially Organized Communities.- Extraction and Preparation of
Listeria monocytogenes
Subproteomes for Mass Spectrometry Analysis.- Extraction and Analysis of Plasmid DNA from
Listeriamonocytogenes
.- Generation of Nonpolar Deletion Mutants in
Listeria monocytogenes
Using the “SOEing” Method.- Mutant Construction and Integration Vector-Mediated Genetic Complementation in
Listeria monocytogenes
.- Internalization Assays for
Listeria monocytogenes
.- Microscopy of Intracellular
Listeriamonocytogenes
in Epithelial Cells.- Control of
Listeria monocytogenes
Biofilms in a Simulated Food Processing Environment.- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP).- Isolation and Evaluation of Anti-
ListeriaLactococcus lactis
from Vegetal Sources.