Teratogenicity Testing

Methods and Protocols
2. Auflage 2024
ISBN: 978-1-0716-3624-4
Verlag: Springer US

Buch, Englisch, 627 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 1390 g

Reihe: Methods in Molecular Biology

This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Félix, Luís

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie

Inhaltsverzeichnis

Updating an Overview of Teratology.- Guidelines on Development Toxicity Tests: Brief Insights.- Development Features on the Selection of Animal Models for Teratogenic Testing.- Virus as Teratogenic Agents.- The Future of the Teratogenicity Testing.- The First Steps on AOPs' Concepts, Development, and Applications in Teratology.- Machine Learning to Predict Teratogenicity: Theory and Practice.- Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity.- Developmental Toxicity Using the Rat Whole Embryo Culture.- A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs.- Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry.- Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo.- Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds.- Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects.- Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure.- Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster.- Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb.- Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb.- Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX.- Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing.- Angiogenesis Assay for Live and Fixed Zebrafish Larvae.- High-Resolution Respirometry for the Assessment of Teratogenic Chemicals.- A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae.- Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos.- Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish.- Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response.- Alcian Blue Staining for Chondrocranium Development in Zebrafish.- Protocol of Geometric Morphometrics for Teratogenicity Testing.- Biochemical Markers for Liver Injury in Zebrafish Larvae.- Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae.- Metabolomic Fingerprint Assay in Zebrafish Embryos.- Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae.- A Chromogenic Quantification of Protein Expression in Zebrafish Larvae.- Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model.- Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos.- Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos.- Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos.- Cell-Free DNA and Next-GenerationSequencing for Prenatal Diagnosis.