E-Book, Englisch, Band 1145, 255 Seiten, eBook
Reihe: Methods in Molecular Biology
Methods and Protocols
E-Book, Englisch, Band 1145, 255 Seiten, eBook
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-0446-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Crop Breeding: Methods and Protocols
presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible,
Crop Breeding: Methods and Protocols
will help in expanding the use of molecular technologies for the creation of tomorrow’s crop varieties.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
Defining a Genetic Ideotype for Crop Improvement.- From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding.- Temperature Switch PCR (TSP) – A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms.- Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers.- Genotyping by High-Resolution Melting Analysis.- Bi-Allelic SNP Genotyping Using the TaqMan® Assay.- SNP Genotyping: The KASPTM Assay.- Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding.- OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs.- Genomic Selection in Plant Breeding.- Simulated Breeding with QU-GENE Graphical User Interface.- The Control of Recombination in Wheat by Ph1 and Its Use in Breeding.- TILLING for Plant Breeding.- In vitro Culture for Doubled Haploids: Tools for Molecular Breeding.- Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin.- Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.).- qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number.- High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics.
