Fessner / Anthonsen Modern Biocatalysis

Stereoselective and Environmentally Friendly Reactions
1. Auflage 2008
ISBN: 978-3-527-62384-6
Verlag: Wiley-VCH
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 376 Seiten, E-Book

Das Gesamtgebiet der Biokatalyse mit allen seinen Facetten -- Mikrobiologie, Enzymologie, Molekularbiologie, Strukturbiologie, organische Chemie -- wird in diesem interdisziplinär angelegten Werk beleuchtet; insbesondere geht es um enzymatische Katalysen und Ganzzell-Katalysen. Ein Schwerpunkt liegt dabei auf der Entwicklung hochselektiver, umweltfreundlicher Prozesse zur Synthese wichtiger Verbindungsklassen.

Autoren/Hrsg.

Fessner, Wolf-Dieter

Anthonsen, Thorleif

Inhaltsverzeichnis

FLUORESCENCE ASSAYS FOR BIOTRANSFORMATIONS

Introduction

Alcohol Dehydrogenases (ADHs) and Aldolases

Lipases and Esterases

Other Hydrolases

Baeyer-Villigerases

IMMOBILIZATION AS A TOOL FOR IMPROVING ENZYMES

Introduction

Adsorption/Electrostatic Interactions

Encapsulation

Covalent Binding/Cross-Linking

CONTINUOUS-FLOW MICROCHANNEL REACTORS WITH SURFACE-IMMOBILIZED BIOCATALYSTS

Introduction

Biocatalytic Synthesis Using Microreaction Technology with Free and Immobilized Enzymes

Novel Microfluidic Immobilized Enzyme Reactors

Enzymatic Hydrolysis of Lactose

Biocatalytic Process Intensification Using Microreaction Technology

ACTIVITY AND STABILITY OF PROTEASES IN HYDROPHILIC SOLVENTS

Introduction

Activity and Selectivity of Proteases in Synthesis of Carbohydrate Fatty Acid Esters

Enzyme Stability and Conformation

Solvent Engineering

IMPORTANCE OF ENZYME FORMULATION FOR THE ACTIVITY AND ENANTIOSELECTIVITY OF LIPASES IN ORGANIC SOLVENTS

Introduction

Lipase Formulations and their Activity and Enantioselectivity in Neat Organic Solvent

Why do Additives Affect the Activity and Enantioselectivity of Lipases in Organic Solvent?

DIRECT ESTERIFICATION WITH DRY MYCELIA OF MOLDS: A (STEREO)SELECTIVE, MILD AND EFFICIENT METHOD FOR OBTAINING STRUCTURALLY DIVERSE ESTERS

Mycelia and Biotransformations in Organic Media

Screening and Microbiological Aspects

Production of Acetate

Stereoselective Esterifications of Racemic Alcohols

Stereoselective Esterifications of Racemic Carboxylic Acids

Partition Phenomena and Equilibrium of Esterification Reactions

FACTORS AFFECTING ENANTIOSELECTIVITY: ALLOSTERIC EFFECTS

How to Provide Enantiopure Compounds

Factors Affecting the Enantiomeric Ratio E

Asymmetrization of Prochiral Compounds

KINETIC RESOLUTION OF SEC-ALCOHOL IN NON-CONVENTIONAL MEDIA

Introduction

SCFs - Replacement for Organic Solvents in Biocatalysis

Effect of Pressure

Effect of the Acyl Donor/Alcohol Molar Ratio

ILs - Solvents for Sustainable Technology in Biocatalysis

ILs, Based on the N,N'-Dialkylimidazolium Cations as Reaction Media

ILs/SCFs Biphasic Systems as Promising Media for Biocatalysis

The [bmim][PF6]/SC-CO2 System as a Reaction Media

Effect of Acyl Donor Concentration

STRATEGIES FOR THE BIOCATALYTIC LIPOPHILIZATION OF PHENOLIC ANTIOXIDANTS

Introduction

Materials and Methods

Results and Discussion

BIOCATALYSIS APPLIED TO THE SYNTHESIS OF NUCLEOSIDE ANALOGS

Introduction

Chemoenzymatic Modification of the Sugar

Resolution and Anomeric Separation

Biotransformations that Modify the Base

Transglycosylation for the Synthesis of Nucleosides

EFFICIENT FRUCTOOLIGOSACCHARIDE SYNTHESIS WITH A FRUCTOSYLTRANSFERASE FROM ASPERGILLUS ACULEATUS

Introduction

Purification of Fructosyltransferase in Pectinex Ultra SP-L

Properties of Fructosyltransferase from A. aculeatus

Immobilization of Fructosyltransferase from A. aculeatus

Fructooligosaccharide Production Using Sugar Beet Syrup and Molasses

HYDANTOIN RACEMASE: THE KEY ENZYME FOR THE PRODUCTION OF OPTICALLY PURE ALPHA-AMINO ACIDS

Introduction

Search for New Hydantoin Racemases and Molecular Characterization

Biochemical Characterization of Hydantoin Racemase Enzymes

Substrate Enantioselectivity and Kinetic Analysis of Hydantoin Racemases

Proposal for a Reaction Mechanism of Hydantoin Racemase Enzymes

Design of a Tailormade Recombinant Biocatalyst Including Hydantoin Racemase Enzymes for Optically Pure D-Amino Acid Production

CHEMO-ENZYMATIC DERACEMIZATION METHODS

Introduction

Deracemization Methods for Alpha- and Beta-Hydroxy Acids

Deracemization of Alpha-Hydroxy Nitriles

Deracemization of Alpha-Amino Acids

Useful Enzymes for Deracemization Methods

NITRILASES FROM FILAMENTOUS FUNGI

Introduction

Distribution and Evolutionary Relationship of Fungal Nitrilases

Structural Properties

Catalytic Properties

NITRILASE- AND NITRILE HYDRATASE-CATALYZED ENANTIOSELECTIVE PREPARATION OF NON-PROTEINOGENIC AMINO ACIDS

Introduction

Nitrilase Hydratase/Amidase Biotransformations

Nitrilase Biotransformations

NITRILASES IN

Über Autor(innen)

Wolf-Dieter Fessner obtained his PhD from the University of Freiburg, following which he carried out postdoctoral research at Harvard University with George Whitesides and at the University of Southern California with George Olah. Since 1998 he has been Full Professor of Organic Chemistry at the Technische Universität Darmstadt. His primary research interest is focused on the development of practical methods for enzymatic carbon-carbon bond formation and oligosaccharide synthesis, and on the interface between chemical and biological catalysis.

 

Thorleif Anthonsen is Professor of Organic Chemistry at The Norwegian University of Science and Technology, NTNU, in Trondheim. He has been a post doc/visiting scholar at Glasgow University, UK, at UCLA Los Angeles, with Donald Cram at MIT Cambridge, USA, with Alexander Klibanov and at Cytel Corporation, San Diego. He has led the biocatalysis group at NTNU whose research focused on synthesis of enantiopure compounds directed towards production of chiral drugs. In 2001 he initiated COST action D25, Applied Biocatalysis, which he also chaired from beginning to end. Professor Anthonsen's primary research interests are natural product chemistry and biocatalysis in organic chemistry.