E. coli

Shiga Toxin Methods and Protocols
1. Auflage. Softcover version of original hardcover Auflage 2003
ISBN: 978-1-61737-264-3
Verlag: Humana Press

Buch, Englisch, Band 73, 352 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 527 g

Reihe: Methods in Molecular Medicine

Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods.

Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of ist toxicity.

Zielgruppe

Research

Autoren/Hrsg.

Ebel, Frank

Philpott, Dana

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Medizinische Mikrobiologie & Virologie
• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Biowissenschaften Bakteriologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections: An Overview
Mohamed A. Karmali

Methods for Detection of STEC in Humans: An Overview
James C. Paton and Adrienne W. Paton

Serological Methods for the Detection of STEC Infections
Martin Bitzan and Helge Karch

Detection and Characterization of STEC in Stool Samples Using PCR
Adrienne W. Paton and James C. Paton

Molecular Typing Methods for STEC
Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, and Sunao Iyoda

STEC in the Food Chain: Methods for Detection of STEC in Food Samples
Michael Bülte

STEC as a Veterinary Problem: Diagnostics and Prophylaxis in Animals
Lothar H. Wieler and Rolf Bauerfeind

Cellular Microbiology of STEC Infections: An Overview
Frank Ebel and Dana Philpott

Analysis of Pathogenicity Islands of STEC
Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, and Jörg Hacker

Generation of Isogenic Deletion Mutants of STEC
Soudabeh Djafari, Nadja D. Hauf, and Judith F. Tyczka

Generation of Monoclonal Antibodies Against Secreted Proteins of STEC
Kirsten Niebuhr and Frank Ebel

Microscopic Methods to Study STEC: Analysis of the Attaching and Effacing Process
Stuart Knutton

Detection and Characterization of EHEC-Hemolysin
Herbert Schmidt and Roland Benz

Shiga Toxin Receptor Glycolipid Binding: Pathology and Utility
Clifford A. Lingwood

Methods for the Purification of Shiga Toxin 1
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood

Methods for the Identification of Host Receptors for Shiga Toxin
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood

Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Frédéric Mallard and Ludger Johannes

Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin
Jae H. Kim

Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways
Nicola L. Jones

Interaction of Shiga Toxin with Endothelial Cells
Martin Bitzan and D. Maroeska W. M. te Loo

Shiga Toxin Interactions with the Intestinal Epithelium
Cheleste M. Thorpe, Bryan P. Hurley, and David W. K. Acheson

Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes
Christian Menge

Animal Models for STEC-Mediated Disease
Angela R. Melton-Celsa and Alison D. O'Brien

Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC
Florian Gunzer, Isabel Hennig-Pauka, Karl-Heinz Waldmann, and Michael Mengel

Bovine Escherichia coli O157: H7 Infection Model
Evelyn A. Dean-Nystrom

Index