DNA Damage Detection In Situ, Ex Vivo, and In Vivo

Methods and Protocols
2011
ISBN: 978-1-60327-408-1
Verlag: Humana Press

Buch, Englisch, Band 682, 313 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 823 g

Reihe: Methods in Molecular Biology

Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Didenko, Vladimir V.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Onkologie, Krebsforschung
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Embryologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pathologie, Cytopathologie, Histopathologie

Inhaltsverzeichnis

DNA Damage Detection In Situ, Ex Vivo and In Vivo: Methods and Protocols
Vladimir V. Didenko, Editor
Table of Contents
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Table of Contents

Preface

Contributors

Part 1. DETECTION IN TISSUE SECTIONS
1 In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques. Deryk T. Loo
2 Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage. Alexander E. Kalyuzhny
3 EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level. Antonio Migheli
4 In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase. Vladimir V. Didenko
5 In Situ Ligation: a Decade and a Half of Experience. Peter J. Hornsby and Vladimir V. Didenko
6 In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections. Vladimir V. Didenko
7 5’OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach. Vladimir V. Didenko

Part 2. DETECTION IN CELL CULTURES
8 Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry. Zbigniew Darzynkiewicz and Hong Zhao
9 Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis. Zbigniew Darzynkiewicz, Piotr Pozarowski, Brian W. Lee and Gary L. Johnson
10 Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Sergey Shaposhnikov, Preben D. Thomsen, and Andrew R. Collins
11 Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH). José Luis Fernández, Dioleyda Cajigal, Jaime Gosálvez
12 Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells. Jennifer Guerrero-Santoro, Arthur S. Levine, and Vesna Rapic-Otrin

Part 3. DETECTION IN LIVE TISSUES, BLOOD, URINE, SPERM
13 Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at