Co-Immunoprecipitation Methods for Brain Tissue

1. Auflage 2019
ISBN: 978-1-4939-8984-3
Verlag: Springer

Buch, Englisch, Band 144, 166 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 547 g

Reihe: Neuromethods

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Borroto-Escuela, Dasiel O.

Odagaki, Yuji

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Interdisziplinäres Wissenschaften Wissenschaften Interdisziplinär Neurowissenschaften, Kognitionswissenschaft
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Biowissenschaften Neurobiologie, Verhaltensbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft

Inhaltsverzeichnis

Table of Contents 
1. Co-Immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain TissueKazunori Namba and Hiroki Kaneko
2. Co-Immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor a at Postsynaptic Density in Brain Tissue Gen Murakami and Suguru Kawato
3. Use of Co-Immunoprecipitation to Study Conformation-Specific protein Interactions of Oligomeric a-Synuclein AggregatesCristine Betzer, Rikke Hahn Kofoed, Poul Henning Jensen
4. Using Co-Immunoprecipitation and Shotgun Mass Spectrometry for protein-Protein Interaction Identification in Cultured Human OligodencrocytesBradley Smith, Daniel Martins-de-Souza, and Mariana Fioramonte
5. Co-Immunoprecipition Analysis of GPCR Complexes in the Central Nerve SystemYuji Kamikubo and Takashi Sakurai
6. The Histoblot Technique: A Reliable Approach to Analyse Expression Profile of proteins and to Predict their Molecular AssociationCarolina Aguado and Rafael Luján
7. Co-Immunoprecipitation Assay to Investigate the Interaction Strength between Synaptic Protein using COS-7 CellsSosuke Yagishita
8. Guanosine-5’-O-(3-[35S]thio)triphosphate ([35S]GTPS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-G Antibody in Mammalian Brain MembranesYjui Odagaki
9. Co-Immunoprecipitation as a Useful Tool for Detection of G protein-Coupled Receptors OligomersKirill Shumilov, Alejandra Valderrama-Carvajal, Maria Garcia-Bonilla, and Alicia Rivera
10. Isolation and Detection of G Protein–Coupled receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation using a Combined Brain Subcellular Fractionation/Co-Immunoprecipitation (co-IP) ProceduresDasiel O. Borroto-Escuela, Manuel Narvaez, Martina Zannoni, Chiara Contri, Minerva Crespo-Ramírez, Michael di Palma, Patrizia Ambrogini, Daily Y. Borroto-Escuela, Ismel Brito, Mariana Pita-Rodríguez, Ismael Valladolid-Acebes, Miguel Pérez de la Mora and Kjell Fuxe
11. Co-Immunoprecipitation of Membrane-Bound Receptors from Subsynaptic CompartmentsWilber Romeo-Fernandez, Maria Garcia-Barcelo, and Yunis Perez-Betancourt
12. Co-Immunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita during AestivationDaily Y. Borroto-Escuela, Idania Herández-Ramos, Kjell Fuxe and Dasiel O. Borroto-Escuela
13. Co-Immunoprecipitation (co-IP) of G Protein-Coupled Receptors (GPCR)-Receptor Tyrosine Kinases (RTK) Complexes from the Dorsal Hippocampus of the Rat BrainMichael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini, Kjell Fuxe, Patrizia Ambrogini and Dasiel O. Borroto-Escuel