Plant Hormones

Methods and Protocols
2. Auflage 2009
ISBN: 978-1-61737-930-7
Verlag: Humana Press

Buch, Englisch, Band 495, 146 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 248 g

Reihe: Methods in Molecular Biology

The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and cutting edge, Plant Hormones: Methods and Protocols, Second Edition is an essential guide for all plant scientists who are facing the complex and challenging questions that will determine the future of this vital field.

Zielgruppe

Research

Autoren/Hrsg.

Bonetta, Dario

Cutler, Sean

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik Pflanzenphysiologie, Photosynthese

Inhaltsverzeichnis

Plant Hormones: Methods and Protocols, Second Edition
S. Cutler and D. Bonetta, eds.

Table of Contents

Preface

Contributors

1. Using reverse genetics to develop small knockout collections for specific biological questions.
J. Northey and P. McCourt

2. Visualizing auxin transport routes in Arabidopsis leaf primordia.
D. Marcos and T. Berleth

3. Bioinformatic analysis of hormone microarray data.
G.W. Bassel and N.J. Provart

4. Measurement of plant hormones by liquid chromatography-mass spectrometry.
S.J. Owen and S.R. Abrams

5. Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry.
M. Okamoto, A Hanada, Y Kamiya, S Yamaguchi and E Nambara

6. Pull-down assays for plant hormone research.
S. Kepinski

7. Binding assays for brassinosteroid receptors.
A. Caño-Delgado and Z-Y Wang

8. Binding assays for abscisic acid receptors.
F.A. Razem and R.D. Hill

9. Cytokinin sensing systems using microorganisms.
M. Higuchi, T. Kakimoto and T. Mizuno

10. Hormone-binding assay using living bacteria expressing eukaryotic receptors.
G.A. Romanov and S.N. Lomin

11. Discovery of plant microRNAs and short-interfering RNAs by deep parallel sequencing.
P. Chellappan and H. Jin

12. The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana
L. Norambuena, G.R. Hicks and N.V. Raikhel