Berliner Biological Magnetic Resonance

Volume 5
Erscheinungsjahr 2012
ISBN: 978-1-4615-6543-7
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 5, 305 Seiten, eBook

Reihe: Biological Magnetic Resonance

Judging from the articles published in Biochemistry, magnetic resonance techniques (NMR and ESR) are now among the most popular methods in biochemical research. The series Biological Magnetic Resonance, the fifth volume of which we are proudly presenting, is intended to provide authori tative coverage of topics of current interest. Previous volumes have covered a number of aspects in a thorough and pedagogical fashion rarely found in other publications in this field. Continuing to fulfill the mission of the series, this volume presents a chapter by Baxter, Mackenzie, and Scott on the applications of carbon-13 NMR spectroscopy in investigations of methabolic pathways in vivo. Blom berg and Ruterjans give a comprehensive summary of the use of nitrogen-15 NMR in studies of systems of biological interest. Phosphorus-3I NMR investigations of enzyme systems are described by Rao. Tsai and Bruzik outline the principles of and summarize the state-of-the-art advances in the 18 use of oxygen isotopes e 70 and 0) in phosphorus-3I and oxygen-17 NMR studies of biophosphates. Lipid-protein interactions as reflected in ESR and NMR data are discussed by Devaux. We wish to thank the authors for their cooperation in maintaining the and continued high standards of the series.

Zielgruppe

Research

Autoren/Hrsg.

Berliner, Lawrence

Inhaltsverzeichnis

1 CMR as a Probe for Metabolic Pathways in vivo.- 1. Introduction.- 2. Constraints.- 3. CMR Studies of Metabolic Pathways.- 4. CMR of Metabolism in Whole Organs and Organisms.- 5. Conclusions.- References.- 2 Nitrogen-15 NMR in Biological Systems.- 1. Introduction.- 2. Properties of the Nitrogen NMR Probe.- 3. Experimental Techniques.- 4. Amino Acids and Peptides.- 5. Proteins.- 6. Nucleosides, Nucleotides, and Nucleic Acids.- 7. Coenzymes, Antibiotics, and Other Biologically Important Molecules.- 8. 15N NMR of Living Cells and Cell Walls.- 9. Concluding Remarks.- Notation.- References.- 3 Phosphorus-31 Nuclear Magnetic Resonance Investigations of Enzyme Systems.- 1. Introduction.- 2. Basic Considerations.- 3. Experimental Results on Specific Enzyme Systems.- 4. Concluding Remarks.- References.- 4 NMR Methods Involving Oxygen Isotopes in Biophosphates.- 1. Introduction.- 2. 18O Isotope Shifts in 31P NMR, S31P_18O.- 3. 17O NMR of Biophosphates.- 4. 31P(17O)NMR.- 5. Applications in the Stereochemistry of Enzyme Reactions at Phosphorus.- 6. Other Biochemical Applications.- Notation.- References.- 5 ESR and NMR Studies of Lipid-Protein Interactions in Membranes.- 1. Magnetic Resonance and Lipid-Protein Interactions: An Introduction.- 2. Rotational Diffusion of Membrane Proteins as Influenced by Lipids: Use of Saturation Transfer ESR (ST-ESR).- 3. Determination of the “ Average Viscosity” of Biological Membranes by Conventional ESR.- 4. “ Boundary Lipids “ of Intrinsic Proteins : Electron Spin Resonance Studies.- 5. Boundary Lipids of Intrinsic Proteins: 2H NMR Studies.- 6. Boundary Lipids of Intrinsic Proteins: NMR Studies with 1H, 13C, 19F, or 31P.- 7. Specific Interactions between Lipids and Membrane Proteins. ESR and NMR Studies. Towards an Understanding of theDiversity of Lipid Composition.- 8. Concluding Remarks and Future Prospects.- References.