Single-Molecule Science

Erscheinungsjahr 2022
ISBN: 978-1-108-42336-6
Verlag: Cambridge University Press

Buch, Englisch, 550 Seiten, Format (B × H): 175 mm x 250 mm, Gewicht: 484 g

Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology.

Autoren/Hrsg.

Appasani, Krishnarao

Appasani, Raghu Kiran

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Bildgebende Verfahren, Nuklearmedizin, Strahlentherapie Radiologie, Bildgebende Verfahren
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Physik Elektromagnetismus Mikroskopie, Spektroskopie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biowissenschaften DNA und Transgene Organismen

Inhaltsverzeichnis

Preface; I. Super Resolution Microscopy and Molecular Imaging Techniques to Probe Biology: 1. Introduction on Single Molecule Science Krishnarao Appasani and Raghu K. Appasani; 2. One molecule, two molecules, red molecules, blue molecules: Methods for quantifying localization microscopy data George Patterson and Gaetan G. Herbomel; 3. Muti-scale fluorescence imaging Ruben Bulkescher, Inn Chung, Jan Philipp Eberle, Holger Erfle, Manuel Gunkel, Jürgen Reymann, Karsten Rippe, Guido Sauter, Ronald Simon and Vytaute Starkuviene; 4. Long-read single-molecule optical maps Assaf Grunwald, Yael Michaeli and Yuval Ebenstein; II. Protein Folding, Structure, Confirmation and Dynamics: 5. Single-molecule mechanics of protein nanomachines Gabriel Žoldák and Katarzyna Tych; 6. Post-translational Protein Translocation Through Membranes at the Single Molecule Level Diego Quiroga-Roger, Hilda M. Alfaro-Valdés and Christian A.M. Wilson Moya; III. Mapping DNA Molecules at Single Molecule Level: 7. Observing Dynamic states of single molecule DNA and Proteins using Atomic Force Microscope Jingqiang Li, Sithara Wijeratne and Ching-Hwa Kiang; 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification Sourav Mishra, Yoonhee Lee and Joon Won Park; IV. Single Molecule Biology to Study Gene Expression: 9. Single molecule detection in the study of gene expression Vipin Kumar, Simon Leclerc and Yuichi Taniguchi.

Über Autor(innen)

Appasani, Krishnarao
Krishnarao Appasani is the Founder and Chief Executive Officer of GeneExpression Systems, a global conference-producing organisation focusing on biomedical and physical sciences. He is also an elected Life Fellow of the Royal Society of Biologists, London, UK.

Appasani, Raghu Kiran
Raghu Kiran Appasani is a Psychiatrist and Neuroscientist. He practices at University of Southern California. He is also the Founder and Chief Executive Officer of The MINDS Foundation.