Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies

1. Auflage 1999
ISBN: 978-0-08-049670-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 313, 580 Seiten, Web PDF

Reihe: Methods in Enzymology

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Autoren/Hrsg.

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 313;10
4;Preface;14
5;Volumes In Series;16
6;Section I: General Methods;35
6.1;Chapter 1. Progress in Antisense Technology: The End of the Beginning;37
6.2;Chapter 2. Basic Principles of Using Antisense Oligonucleotides in Vivo;80
6.3;Chapter 3. Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3' -Capped Phosphodiester Oligonucleotides;90
6.4;Chapter 4. Design of Antisense and Triplex-Forming Oligonucleotides;108
6.5;Chapter 5. Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular and Cellular Biology Protocols;129
6.6;Chapter 6. Evaluation of Antisense Mechanisms of Action;169
6.7;Chapter 7. Physiology and Molecular Biology Brought to Single-Cell Level;177
6.8;Chapter 8. Antisense Properties of Peptide Nucleic Acid;190
6.9;Chapter 9. Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide;198
6.10;Chapter 10. Gene Switching: Analyzing Broad Range of Mutations Using Steric Block Antisense Oligonucleotides;208
6.11;Chapter 11. Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation Arrest;223
6.12;Chapter 12. Purification of Antisense Oligonucleotides;237
6.13;Chapter 13. Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phosphonates;260
6.14;Chapter 14. Intracellular Distribution of Digoxigenin-Labeled Phosphorothiate Oligonucleotides;291
6.15;Chapter 15. Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression;302
7;Section II: Methods of Delivery;319
7.1;Chapter 16. Determination of Cellular Internalization of Fluorescent Oligonucleotides;321
7.2;Chapter 17. Intrabody Tissue-Specific Delivery of Antisense Conjugates in Animals: Ligand–Linker Antisense Oligomer Conjugates;331
7.3;Chapter 18. Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Applications;356
7.4;Chapter 19. In Vitro Transport and Delivery of Antisense Oligonucleotides;376
7.5;Chapter 20. In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipofection: Application of Antisense Technique to Growth Suppression of Experimental Glioma;393
7.6;Chapter 21. Preparation and Application of Liposome-Incorporated Oligodeoxynucleotides;406
7.7;Chapter 22. Cell-Specific Optimization of Phosphorothioate Antisense Oligodeoxynucleotide Delivery by Cationic Lipids;422
8;Section III: RNA Studies;433
8.1;Chapter 23. Intracellular Expression and Function of Antisense Catalytic RNAs;435
8.2;Chapter 24. Selective Degradation of Targeted mRNAs Using Partially Modified Oligonucleotides;454
8.3;Chapter 25. Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucleotide-Targeted Degradation in Frog Oocytes;470
8.4;Chapter 26. Inactivation of Gene Expression Using Ribonuclease P and External Guide Sequences;476
8.5;Chapter 27. Disruption of mRNA-RNP Formation and Sorting to Dendritic Synapses by Antisense Oligonucleo- tides;490
8.6;Chapter 28. Antisense RNA and DNA in Escherichia coli;501
8.7;Chapter 29. Utilization of Properties of Natural Catalytic RNA to Design and Synthesize Functional Ribozymes;519
8.8;Chapter 30. Antisense Oligonucleotides and RNAs as Modulators of pre-mRNA Splicing;540
8.9;Chapter 31. Selective RNA Cleavage by Isolated RNase L Acti- vated with 2–5A Antisense Chimeric Oligonucleotides;556
9;Author Index;569
10;Subject Index;601